Qalabka ElasFree Midi Plasmid

Si dhakhso ah loo nadiifiyo qaddarka dhexdhexaadka ah ee dhiiga translas-ka aan lahayn endotoxin DNA plasmid.

Qalabka EndoFree Midi Plasmid wuxuu qaataa farsamo xayeysiis ah xuubka silica si si hufan oo si gaar ah loogu xiro DNA -da plasmid -ka. Buffer -ka gaarka ah EBT iyo Filtration CS1 ayaa si wax ku ool ah uga saari kara wasakhda sida endotoxin iyo borotiinka. Habka soo saaridda oo dhan waxay qaadataa 1 saac oo keliya, taas oo ku habboon oo degdeg ah.

Bisad. Maya	Cabbirka xirxirida
4992853	10 preps

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Faahfaahinta Alaabta

Socodka shaqada

Tusaalaha Tijaabada

Calaamadaha alaabta

Astaamaha

■ Ku habboon ka-soo-saaridda plasmids-ka 50-100 ml dhexdhexaadka dhaqanka bakteeriyada.
Plazmid -ka la sifeeyay waxaa ku jira endotoxin aad u hooseeya wuxuuna buuxin karaa shuruudaha gudbinta oo tayo sare leh.
■ Way fududahay in lagu shaqeeyo hab ka -saarid endotoxin oo gaar ah oo degdeg ah.

Codsiyada

DNA -da la nadiifiyey ayaa lagu dabaqi karaa tijaabooyinka bayoolaji molecular iyo tijaabooyinka bayoolaji unugyada sida xaddidaadda dheef -shiidka endonuclease, isku xigxiga, is -beddelka, gudbinta, microinjection, iwm.

Waxsoosaarka Plasmid

Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)

Hore: TIANprep Rapid Mini Plasmid Kit

Xiga: TIANprep Rapid N96 Kit Plasmid

	Plasmid pEGFP-C1 waxaa lagu nadiifiyaa EndoFree Midi Plasmid Kit laga bilaabo 50 ml dhexdhexaad ah bakteeriyada dhaqameedka. Feejignaanta plasmid -ka waxaa lagu cabiray Nanodrop oo dhalidda waa la xisaabiyey. Natiijadu waxay muujineysaa in soosaarka plasmid -ka ee EndoFree Mini Plasmid Kit uu ka sarreeyo kan badeecadda laxiriirta ee ka timid Alaab -qeybiye A.
	PEGFP-C1-ka plasmid-ka ah ee laga soo saaray EndoFree Midi Plasmid Kit waxaa loo gudbiyaa 80% unugyada 293T ee isku-darka TIANGEN TIANfect. Muujinta GFP waxaa la ogaadey 24-36 h kadib qaadistii. Natiijadu waxay muujineysaa in hufnaanta gudbinta ay ahayd 80%-90%.