EndoFree Maxi Plasmid Kit

Si loo nadiifiyo DNA-da plasmid-ka ee fasalka gudbinta ee aan endotoxin lahayn.

Qalabka EndoFree Maxi Plasmid wuxuu ku salaysan yahay tiknoolajiyada xuubka silica si loo soo saaro DNA-da plazmid-la'aanta ah ee endotoxin. Qalabkani wuxuu bixiyaa hab ka-saarid endotoxin oo gaar ah si loo abuuro DNA-ga plasmid-ka oo tayo sare leh ee codsiyada hoose. Waxaa loogu talagalay in lagu go'doomiyo DNA-da plasmid-ka ah ee ugu nadiifsan 100-200 ml oo ah dhaqanka bakteeriyada waxaana laga yaabaa inay soo saarto 1.5 mg oo ah fasmid DNA-ga fasalka gudbinta.

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4992194 10 preps

Faahfaahinta Alaabta

Tusaalaha Tijaabada

Calaamadaha alaabta

Astaamaha

Wax-soo-saar degdeg ah oo sarreeya: 200 μg-1.5 mg oo ah plasmid DNA ayaa soo saarta 1 saac oo leh saami aad u sarreeya oo ah qaab dhismeed aad u badan.
Urity Daahirsanaanta sare: DNA-ga plasmid-ka ah ee aadka u sarreeya waxaa helay nidaamka keydinta gaarka ah iyo Spin Column CP6.
Efficiency Wax -ku -oolnimada gudbinta oo aad u fiican: Ku habboon tijaabooyinka gudbinta ee khadadka unugyada badankood.
Applications Codsiyo baaxad leh: Ku habboon dheef -shiidka enzyme -ka xaddidaadda, beddelidda, isku xigxiga, microinjection, aamusnaanta hiddaha iyo tijaabooyinka gudbinta.

Waxsoosaarka Plasmid

Plasmid Yield

Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)


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    Experimental Example Soosaarid plasmid nuqul sare/hoose
    PBR322 plasmid-nuqul hooseeya, oo laga soo saaray 200 ml dhaqanka bakteeriyada iyadoo la adeegsanayo EndoFree Maxi Plasmid Kit, ayaa lagu xardhay 1 ml Buffer TB, oo leh 0.6 μg/μl. PBS plasmid-nuqul-sare ah, oo laga soo saaray 100 ml dhaqanka bakteeriyada iyadoo la adeegsanayo EndoFree Maxi Plasmid Kit, ayaa lagu xardhay 1 ml Buffer TB, oo ay ku urursan tahay 1.2 μg/μl.
    MIV: TIANGEN DNA Marker IV
    pBR322: 2 μl, pBS: 2 μl
    Experimental Example pEGFP oo laga helay EndoFree Maxi Plasmid Kit ayaa si gooni ah loogu gudbiyay khadka unugyada endotoxin-dareen-daran 293T iyo unug-xasaasiyad-xasaasiya endotoxin unugyada Huh7 ee TIANGEN TIANfect. Muujinta GFP waxaa lagu ogaadey 24 saac kadib gudbinta.
    Halkan ku qor fariintaada oo noo soo dir