TIANprep Rapid Mini Plasmid Kit

Si dhakhso ah loo nadiifiyo DNA -ga plasmid -ka ee darajada bayoolaji molikalika iyadoo la adeegsanayo farsamada lisinta alkaline.

Xirmada TIANprep Rapid Mini Plasmid Kit waxaa laga hagaajiyay tiknoolijiyada lysis-dhaqameedka alkaline, kaas oo DNA-ga plasmid-ka oo tayo sare leh lagu nadiifin karo 8 daqiiqo gudahood. Kaydka cusub ee lysis wuxuu u oggolaanayaa in DNA -ga lagu dhejiyo xuubka silica iyadoo ay jirto milix sare. Maaddada ay ka samaysan tahay xuubka silica waa mid gaar ah, aad u hufan oo aad u gaar ah. Qalabkan waxaa loogu talagalay in lagu nadiifiyo ilaa 24 μg DNA-ga plasmid laga bilaabo 1-4 ml dhaqammada habeenkaE.coli. DNA-ga plasmid-ka ah ee lagu nadiifiyey xirmadaan waxaa lagu dabaqi karaa tijaabooyin kala duwan oo joogto ah, sida dheef-shiidka enzyme, PCR, isku-xigxiga, isku-xidhka, is-beddelka, baaritaanka maktabadda, qoraalka faytroolka iyo is-beddelka unugyada dhaqanka hoose ee caadiga ah, iwm.

Bisad. Maya	Cabbirka xirxirida
4992191	50 preps
4992192	200 preps

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■ Degdeg: Nidaam fudud oo leh tillaabooyin aad u yar oo lagu nadiifiyo DNA -ga plasmid -ka saafiga ah 8 daqiiqo oo keliya.
Efficiency Wax -ku -oolnimada sare: Waxaa laga yaabaa inay ka nadiifiso in ka badan 85% DNA -ga plasmid -ka E.coli.
Indicator Tusaha midabka TIANRed waxaa la siiyay si loo xakameeyo loona xusuusto geeddi -socod kasta inta lagu jiro tijaabada si loo hubiyo hufnaanta sare ee is -daahirinta plasmid -ka.

Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)

Hore: TGuide S32 Automatic Nucleic Acid Extractor

Xiga: Qalabka ElasFree Midi Plasmid

Iyada oo leh daaweyn lysate-karti sare leh iyo nidaamka kaydinta oo la hagaajiyay, TIANprep Rapid Mini Plasmid Kit ayaa si sahal ah u heli kara alaab tayo sare leh iyadoo la badbaadinayo waqti badan oo hawlgal ah.

Isbarbar dhigga u dhexeeya TIANprep Rapid Mini Plasmid Kit iyo Supplier A (xirmada guud ee nadiifinta DNA -ga plasmid) iyadoo isla mar la nadiifinaayo isla galaas.

Mugga tusaalaha: 3 ml dhaqanka habeenka E.coli (OD600 = 1.8); Mugga digniinta: 50 μl; Mugga soo -dejinta: 1 μl pBS, 3 μl pET30a, 3 μl pBR322.

Qalabka korontada ayaa lagu sameeyay 6 V/cm muddo 30 daqiiqo ah 1% jel agarose ah. M: TIANGEN DNA Marker IV.

Gunaanad: Agarose gel electrophoresis waxay muujineysaa in TIANprep Rapid Mini Plasmid Kit laga heli karo dhalid la mid ah DNA -ga plasmid -ka ah kaas oo ah xirmada guud ee nadiifinta plasmid 8 daqiiqo gudahood.

TIANprep Rapid Mini Plasmid Kit