■ Ku habboon oo fudud: 96 muunad ayaa la soo saari karaa 2-3 saacadood gudahood.
■ Ku qalabaysan reagent TIANRed oo gaar ah, waxay si cad u tilmaami kartaa heerka kala-qaadista muunadda ee habka soo-saaridda si loo hubiyo DNA-da plasmid-nadaafadeed.
Urity Daahirsanaanta sare: Plazmid-ka aadka u sarreeya ayaa si toos ah loogu isticmaali karaa tijaabooyinka hoose.
Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)
DNA-ga Plasmid waxaa laga soo saaray 1 ml oo maraq Escherichia coli ah (TOP10) maraq (LB dhexdhexaad) iyadoo la adeegsanayo TIANprep Rapid N96 Plasmid Kit, iyo celcelis ahaan 8-10 μg oo ah pBS plasmid oo nadiif ah. 2 µl plasmid ayaa lagu shubay ceel kasta oo ah 1% jel agarose ah, waxaana lagu ogaaday electrophoresis. |
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DNA-ga Plasmid waxaa laga soo saaray 1 ml oo maraq Escherichia coli ah (TOP10) maraq (LB dhexdhexaad) iyadoo la adeegsanayo TIANprep Rapid N96 Plasmid Kit, iyo celcelis ahaan 8-10 μg oo ah pBS plasmid oo nadiif ah. Vector: pBS plasmid; Cadaadis: TOP10. Plazmid -ka waxaa isku xigay ABI. PRISM 3730XL taxanaha DNA |
Tan iyo markii la aasaasay, warshaddeennu waxay horumarinaysay alaabada heerka koowaad ee adduunka iyadoo la raacayo mabda'a
tayo leh marka hore. Alaabooyinkayagu waxay sumcad fiican ka heleen warshadaha iyo aaminaad qiimo leh oo ka dhexaysa macaamiisha cusub iyo kuwii horeba ..