DNA Bisulfite Conversion Kit Featured Image

Qalabka Beddelka DNA Bisulfite

Beddelidda iyo nadiifinta waxaa lagu dhammayn karaa 2 saac, iyada oo heerka beddelidda ilaa 99%.

Qalabka Beddelka DNA Bisulfite waxaa si gaar ah loogu sameeyay beddelka bisulfite DNA ee baaritaanka methylation DNA. Daaweynta shaybaarka DNA -ga iyadoo la adeegsanayo xirmadaan waxaa lagu dhammeyn karaa 2 saacadood, iyo heerka u -beddelka cytosine -ka aan loo baahnayn ee uracil wuxuu ka sarreeyaa 99%. Qalabkani wuxuu qaataa qayb gaar ah oo ah wakiilka difaaca DNA, kaas oo dammaanad qaadaya tayada iyo soo -kabashada DNA -ga kadib beddelidda. Intaa waxaa dheer, xirmadani waxay kaloo isticmaashaa habka ka saarista kooxda aashitada sulphurous acid, taas oo ka dhigaysa habka hawlgalka oo dhan mid sahlan.

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■ Fudud oo degdeg ah: Hanaanka beddelidda iyo nadiifinta waxaa lagu dhammayn karaa 2 saacadood, iyada oo shuruudo hoose u leh aaladaha iyo qalabka, taas oo ku habboon hay'adaha cilmi -baarista heer walba.
Rate Heerka beddelidda oo sarreysa: Heerka u -beddelka cytosine -ka aan la qiyaasi karin ee uracil ee saamiga DNA -ga ayaa ka sarreeya 99%.
Sens Xasaasiyad sare: Qalabkani wuxuu ka shaqayn karaa shaybaarka DNA ee ugu hooseeya 500 pg iyo ilaa 2.5 μg.

Faahfaahinta

Nooca: Beddelka Bisulfite
Muunadda DNA: 500 pg-2.5 μg
Heerka beddelka: > 99%
Waqtiga hawlgalka: ~ 2 saac
Codsiyada: Shaybaarada DNA -da ee lagu sameeyay qalabkan ayaa ku habboon methylation PCR/qPCR gaar ah, isku xigxiga iyo microarray.

Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)

Hore: TIANSeq mRNA Kit Qabashada

Xiga: Qalabka Methylation-specipc PCR (MSP)

Shaxda 1: Heerka u beddelidda “C” oo aan la metelin “U”
Adeegsiga hidde -sidaha aadanaha sida shay tijaabo ah, heerka soo -kabashada ee saamigu wuxuu gaari karaa 65%, iyo saamiga “C” iyo “U” oo aan la qiyaasi karin ayaa gaari kara in ka badan 99%.

Table 1: Conversion rate of unmethylated “C” to “U” Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of unmethylated “C” to “U” can reach more than 99%.