Qalabka Falanqaynta HRM (EvaGreen)

Astaamaha

Resolution Qaraar sare: Qaado midabada cufan ee EvaGreen, oo leh xallinta sare ee qalooca dhalaalka ee gobolka buuxa oo kala saari kara kala duwanaanshaha hal saldhig.
Specific Qeexitaan sare: Adeegso antibody-ka la beddelay ee kuleylka kuleylka ah ee polymerase DNA si loo yareeyo xoojinta aan gaarka ahayn iyo hagaajinta gaar ahaaneed.
Stability Degganaansho sare: Si taxaddar leh loo hagaajiyo nidaamka Buffer wuxuu kordhiyaa xasilloonida qalooca dhalaalka wuxuuna hagaajiyaa isku halaynta natiijooyinka.
Ction Sixitaanka ROX: Rinjiga ROX si gooni ah ayaa loo baakadeeyay, kaas oo u dabacsan in la isticmaalo lana helo natiijooyin sax ah.

Faahfaahinta

Nooca: Antibody modified Taq DNA polymerase, EvaGreen.
Codsiyada: Qoritaanka SNP ee la yaqaan; Baaritaanka SNP ee aan la aqoon; Baadhitaanka hidda -wadayaasha mutant ee aan la garanayn; Falanqaynta Methylation PCR.

Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)

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DNA -ga hidde -wadaha ayaa laga soo saaray 100 μl oo ah saamiga dhiigga aadanaha iyadoo la adeegsanayo TIANamp DNA Kit. 50 ng DNA ayaa loo raray waqtiga dhabta ah ogaanshaha PCR. Marka loo eego maktabadda NCBI SNP, SNP -ga loo yaqaan hiddaha FEN1 (RS 174538) ayaa la xushay oo la ogaaday iyadoo la adeegsanayo Roche LightCycle480. Natiijooyinka waa sida soo socota:

Natiijooyinku waxay muujinayaan in Xidhmada Falanqaynta HRM ay tahay habka falanqaynta aagga SNP ee ku habboon dib -u -noqoshada qalooca dhalaalka ee isla hiddo -wadaha, xallinta cad ee hiddo -wadayaasha kala duwan iyo xukun -xumo.

Macluumaadka SNP: …… CGGAAGAACACGTCG [A/G] CAGGAGCAGGCGCCT …… (Allele Frequency: A = 0.314, G = 0.686)

Horudhaca: 108 bp jajab (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTCCGGTTGTG)