TIANamp N96 Qalabka DNA ee Dhiigga

Nadiifinta wax -soo -saarka sare ee DNA hidde -wadaha dhiigga.

Qalabku wuxuu qaataa tiirka xayeysiinta ee centrifugal oo awood u leh inuu si hufan oo si gaar ah u xiro DNA iyo nidaam kayd oo gaar ah. Waa hawl fudud oo degdeg ah, waxayna muddo gaaban ku dhammayn kartaa soo saaridda DNA -ga 96 saami oo kala duwan. DNA -da hidde -wadaha ah ee la soo saaray waxaa loo adeegsan karaa ogaanshaha hidda -wadaha ama teebaynta unugyada, PCR, dheef -shiid kiimikaad, cirridka koonfureed iyo hawlgallo kale oo tijaabo ah.

Bisad. Maya Cabbirka xirxirida
4992450 4 preps

Faahfaahinta Alaabta

Socodka shaqada

Tusaalaha Tijaabada

Su'aalaha badanaa la isweydiiyo

Calaamadaha alaabta

Astaamaha

Iso Go'doon degdeg ah oo hidde -sideyaasha dhiigga oo tayo sare leh leh.
■ Looma baahna reagent dabiici ah oo sun ah sida phenol/chloroform.
■ Sunta iyo ka -hortagayaasha ayaa gabi ahaanba laga saari karaa, ku habboon codsiga hoose.
Kit Xirmadaan waxaa loo adeegsan karaa meelaha shaqada laga soo saaro ee nucleic acid oo si otomaatig ah loo soo saaro.

Codsiyada

■ PCR iyo qPCR waqtiga-dhabta ah

Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)


  • Hore:
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    Workflow:

    DNA -da hidda -socodka waxaa laga soo saaray 600 µl oo dhiig ah iyadoo la adeegsanayo TIANamp N96 Dhiigga DNA.
    Uruurinta jel agarose waxay ahayd 1%. Electrophoresis waxaa lagu sameeyay 6V/cm 20 daqiiqo. M: kerDNA/Hind III calaamadeeyaha DNA

    Genome DNA was extracted from 600 µl of blood using TIANamp N96 Blood DNA Kit. The concentration of agarose gel was 1%. The electrophoresis was performed under 6V/cm for 20 min. M:λDNA/Hind III DNA Marker

    S: Wax yar ama ma jiro DNA oo sarreysa.

    A1

    A-2 Liisaska oo aan ku filnayn — Shaybaarada si fiican looguma qaso bakhaarka lysis-ka. Waxaa lagu talinayaa in si fiican loo qaso garaaca wadnaha-1-2 jeer. —Lysis unugyada oo aan ku filnayn oo ay sababtay hoos u dhaca hawsha borotiinase K. —Lysis unugyada oo aan ku filnayn ama hoos u dhaca borotiinka sababtuna tahay wakhtiga qubayska diiran oo aan ku filnayn. Waxaa lagu talinayaa in la gooyo cadka qaybo yaryar oo la kordhiyo wakhtiga qubayska si looga saaro dhammaan inta ku hadhay lysate -ka.

    A-3 Xayeysiin DNA oo aan ku filnayn. —Ma jirto ethanol ama boqolkiiba hoose halkii 100% ethanol lagu dari lahaa ka hor inta lysate-ka loo wareejinin tiirka wareegga.

    A-4 Qiimaha pH ee keydinta xaraashka ayaa aad u hooseeya. -U hagaaji pH inta u dhaxaysa 8.0-8.3.

    S: DNA si fiican ugama fiicna tijaabooyinka falcelinta enzymatic -ka hoose.

    Ethanol -ka hadhay ee ugu sarreeya.

    —Waxaa jira kaydka maydhashada haraaga PW ee ugu sarreeya. Ethanol-ka waxaa lagu saari karaa iyada oo la dhexdhigo tiirka wareegga ee 3-5 daqiiqo, kadibna la dhigo heerkulka qolka ama 50 ℃ incubator 1-2 min.

    S: Xaalufka DNA

    A-1 Muunaddu ma aha mid cusub. —Soo saar DNA tijaabo ah oo togan si kontorool ah si loo go'aamiyo in DNA -da sambalku hoos u dhacay.

    A-2 Daaweyn hore oo aan habboonayn. —Waxay sababtay qulqulka dareeraha nitrogen ee xad -dhaafka ah, soo celinta qoyaanka, ama qaddar aad u badan oo saamiga ah.

    S: Sida loo sameeyo u -diyaargarowga soo -saaridda gDNA?

    Saamaynta hore waa inay ku kala duwanaadaan shaybaarka kala duwan. Muunadaha dhirta, hubso inaad si fiican u shiiddo dareeraha nitrogen. Muunadaha xayawaanka, isku -darka ama si fiican ugu shiid nitrogen dareere ah. Shaybaarrada leh derbiyo unug oo ay adag tahay in la jebiyo, sida bakteeriyada G+ iyo khamiirka, waxaa lagu talinayaa in la isticmaalo lysozyme, lyticase ama habab farsamo si loo jebiyo derbiyada unugga.

    S: Waa maxay farqiga u dhexeeya saddexda xirmooyinka soosaarka gDNA ee dhirta 4992201/4992202, 4992724/4992725, 4992709/4992710?

    4992201/4992202 Plant Genomic DNA Kit waxay qaadataa hab ku salaysan tiirka oo u baahan chloroform si loo soo saaro. Waxay si gaar ah ugu habboon tahay shaybaarrada dhirta ee kala duwan, iyo waliba budada qallalan ee dhirta. Xidhmada Dhirta ee Hi-DNAsecure sidoo kale waxay ku salaysan tahay tiirka, laakiin uma baahna soo saaridda phenol/chloroform, taasoo ka dhigaysa mid badbaado leh oo aan sun ahayn. Waxay ku habboon tahay dhirta leh polysaccharides sare iyo maaddada polyphenol. 4992709/4992710 DNAquick Plant System wuxuu qaataa hab dareere ku salaysan. Soo saaridda Phenol/chloroform sidoo kale looma baahna. Nidaamka daahirintu waa mid fudud oo degdeg ah oo aan lahayn xad u leh xaddiga bilowga muunadda, sidaa darteed adeegsadayaashu waxay u hagaajin karaan xaddiga si dabacsan iyadoo loo eegayo shuruudaha tijaabada. Cabbirka weyn ee jajabka gDNA waxaa lagu heli karaa dhalid sare.

    Waa maxay qiyaasta wax -soo -saarka gDNA ee laga soo qaaday 1 ml saamiga dhiigga ee TIANamp Dhiigga DNA?

    DNA -ga hidde -wadaha waxaa laga soo saaray mugga kala duwan ee shay -baarka dhiigga oo dhan ee TIANamp DNA Kit. Natiijooyinka waa sida soo socota. Natiijooyinka waxay ku qoran yihiin tixraac kaliya, natiijooyinka soo -saaridda dhabta ah waxay ku xiran tahay xaaladaha muunadaha.

    faq

    S: 4992207/4992208 iyo 4992722/4992723 ma loo isticmaali karaa in laga soo saaro xinjirrada dhiigga ee DNA?

    Soo -saaridda DNA -da xinjirowga dhiigga ayaa la samayn karaa iyadoo la adeegsanayo reagents -ka lagu bixiyay labadan xirmooyin iyadoo si fudud loogu beddelo hab -maamuuska tilmaamaha gaarka ah ee soo -saaridda xinjirta dhiigga ee DNA. Nuqulka jilicsan ee borotokoolka soo -saarka DNA -ga ee xinjirowga dhiigga ayaa la bixin karaa marka la codsado.

    S: Markaad codsaneyso TIANamp Genomic DNA Kit, sidee loogu kala jebin karaa unugyada cusub xannibaad unug?

    Ku jooji muunadda cusub 1 ml PBS, cusbada caadiga ah ama kaydka TE. Si dhammaystiran u samayso saamiga homogenizer oo soo ururi durdurka xagga hoose ee tuubada adigoo ku shubanaya. Ku tuur dusha sare, oo dib u soo celi roobka 200 μl buffer GA. Nadiifinta DNA ee soo socota ayaa la samayn karaa iyadoo la raacayo tilmaamaha.

    S: Sida loo doorto badeecada soo saaridda DNA -ga laga soo qaaday plasma, serum iyo dheecaanka jirka?

    Si loo nadiifiyo gDNA ee ku jirta plasma, serum iyo shaybaarka dareeraha jirka, TIANamp Micro DNA Kit ayaa lagula talinayaa. Si looga nadiifiyo fayraska gDNA shaybaarka serum/plasma, TIANamp Virus DNA/RNA Kit ayaa lagula talinayaa. Si looga nadiifiyo bakteeriyada gDNA shaybaarka serum iyo plasma, TIANamp Bacteria DNA Kit ayaa lagula talinayaa (lysozyme waa in lagu daraa bakteeriyada togan). Muunadaha candhuufta, Hi-Swab DNA Kit iyo TIANamp Bacteria DNA Kit ayaa lagula talinayaa.

    S: Sida loo doorto xirmooyinka soosaarka gDNA ee shaybaarka fangaska?

    Xidhmada Dhirta ee aan sugnayn ee DNA ama Nidaamka Dhirta DNAquick ayaa lagula talinayaa soo saaridda hidde -sidaha fangaska. Soo-saaridda hidda-wadaha khamiirka, Xirmadda DNA-da ee TIANamp ayaa lagula talinayaa (lyticase waa inay is-diyaarsataa).

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