Conditions Xaaladaha Kaydinta: Qalabkan waxaa lagu kaydin karaa 1 toddobaad heerkulka qolka, 1 maalin 37 ℃, iyo ugu yaraan 1 bil 4 ℃. Shaybaarka unugyada, ku quuso 4 ℃ habeen, una wareeji -20 ℃ ama -80 ℃ kaydinta muddada dheer.
Qabowga iyo Dhalashada soo noqnoqda: Cadka barafoobay ee -20 ℃ ama -80 ℃ waa la qaboojin karaa 20 jeer iyada oo aan saamayn tayada soo saaridda RNA.
Applications Codsiyada hoose: Ka dib marka laga qaado muunadda RNAstore Reagent, wadarta RNA waxaa soo saari kara TIANGEN's TRNzol, RNAprep Pure, RNAsimple reagents iyo xirmooyin.
RNAstore wuxuu ku habboon yahay shaybaarka unugyada cusub.
RNAstore kuma habboona shaybaarka unugyada dhirta.
Ratio Saamiga caddadka shaybaarka unugyada iyo RNAstore Reagent waa inay ahaadaan ugu yaraan 1:10 (tusaale ahaan, 100 mg oo nudaha ah, ugu yaraan 1 ml RNAstore ayaa loo baahan yahay).
■ Dhumucda dhinac kasta oo ka mid ah saamiga waa inaysan ka badnaan 0.5 cm si loo hubiyo in RNAstore -ku uu si dhakhso ah u geli karo nudaha.
Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)
Qalabka: 15 mg Beerka jiirka Habka: 0.5 g unugyada beerka jiirka (oo lagu kaydiyay RNAstore Reagent) ayaa lagu kaydiyay 37 ℃, heerkulka qolka iyo 4 ℃ siday u kala horreeyaan. Wadarta RNA oo ka timid 15 mg shaybaarka unugyada beerka jiirka ee lagu kaydiyey heerkul kala duwan ayaa la go'doomiyey iyadoo la adeegsanayo TRNzol Reagent (Cat. Maya. 4992730). Natiijooyinka: Fadlan fiiri sawirka korontada jel agarose jel. 2-4 μl of 100 μl eluates ayaa lagu raray layn kasta. C (xakameyn togan): muunad nudaha si toos ah loogu keydiyey -80 ℃. Qalabka korontada ayaa lagu sameeyay 6 V/cm muddo 30 daqiiqo ah 1% agarose. |
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Qalabka: 15 mg Beerka jiirka Habka: 0.5 g unugyada beerka jiirka (oo lagu kaydiyay RNAstore Reagent) ayaa freezethawed loogu talagalay 5, 10, 15 iyo 20 jeer siday u kala horreeyaan. Wadarta RNA oo ka timid 15 mg shaybaarka unugyada beerka jiirarka barafoobay ee waqtiyo kala duwan ayaa la go'doomiyey iyadoo la adeegsanayo TRNzol Reagent (Cat. Maya. 4992730). Natiijooyinka: Fadlan fiiri sawirka korontada jel agarose jel. 2-4 μl of 100 μl eluates ayaa lagu raray layn kasta. C (xakameyn togan): muunad nudaha si toos ah loogu keydiyey -80 ℃. 5, 10, 15, 20: waqtiyada barafka barafoobay. Qalabka korontada ayaa lagu sameeyay 6 V/cm muddo 30 daqiiqo ah 1% agarose. |
Tan iyo markii la aasaasay, warshaddeennu waxay horumarinaysay alaabada heerka koowaad ee adduunka iyadoo la raacayo mabda'a
tayo leh marka hore. Alaabooyinkayagu waxay sumcad fiican ka heleen warshadaha iyo aaminaad qiimo leh oo ka dhexaysa macaamiisha cusub iyo kuwii horeba ..