■ Ugu Dhaqsaha badan: Antibody ayaa wax laga beddelay polymerase Anti Taq DNA, oo la kaashanaya nidaamka keydinta degdegga ah ee gaarka ah, keydinta ilaa 60% waqtiga falcelinta iyo noqoshada ugu dhaqsaha badan SYBR Green reagent ee suuqa hadda.
Cap Awoodda cod -weyne oo xoog leh: Calaamadda iftiiminta iftiinka dhaadheer ee xooggan, natiijooyin sax ah oo lagu kalsoonaan karo.
Stability Xasilooni Wanaagsan: Xasiliyaha PCR -ga gaarka ah iyo wax -ku -darka ayaa lagu daray Buffer, taasoo ka dhigaysa natiijada mid xasilloon, ku -celcelis ah, sax ah oo lagu kalsoonaan karo.
Id Aad baa loogu dabaqi karaa: Ma aha oo kaliya ku habboon aaladaha PCR -ga waqtiga dhabta ah ee degdegga ah, laakiin sidoo kale waxay ku habboon tahay aaladaha PCR -ga ee caadiga ah ee caadiga ah.
Ction Sixitaanka ROX: Midabka ROX ayaa si gooni ah loo baakadeeyay, kaas oo u dabacsan in la isticmaalo oo hubin kara natiijooyin sax ah.
Nooca: Antime-modified polymerase DNA-bilow-kulul, SYBR Green I
Waqtiga hawlgalka: ~ 30 daqiiqo
Araajida: qPCR dheeha ku salaysan oo loogu talagalay ogaanshaha hidda-wadaha ee DNA ama falanqaynta muujinta qaraabada ee shaybaarka cDNA ee noocyada kala duwan
Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)
Awoodda cod-weyne oo xoog leh- Calaamadda fluorescence oo xoog badan Sida ugu dhaqsaha badan-Hagaajinta hufnaanta tijaabada, dheerayso nolosha adeegga aaladda iyo badbaadinta tamarta Taq Polymerase DNA si dhaqso leh ayaa loo dhaqaajin karaa, iyadoo la hubinayo in alaabooyinka FastFire ay noqdaan kuwa ugu dhaqsiyaha badan SYBR Green reagent suuqa hadda leh nidaam Buffer dhakhso u gaar ah si loo wanaajiyo hufnaanta tijaabada. |
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Awoodda cod-weyne oo xoog leh- Calaamadda fluorescence oo xoog badan Calaamadda fluorescence-ka la weyneeyay ayaa ka xoog badan (awoodda wax-qabadku waa xoog badan tahay), oo leh qalooc-weyne sii-dheereyn heer sare ah, dareen sare leh, oo si sax ah oo tiro leh u ogaan kara hidd-sidaha bartilmaameedka ee template-ka-fiirsiga hooseeya. Calaamadda ogaanshaha badeecadda la xiriirta ee ka timid Alaab -qeybiye T ayaa daciif ah, qiimihii CT -gu waa gadaal, taasoo keeni karta natiijooyin aan sax ahayn. |
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96-tijaabooyin si isku mid ah u barbar socda ayaa muujinaya in reagent-ku uu leeyahay xasillooni xoog leh iyo soo noqnoqosho wanaagsan. |
Tan iyo markii la aasaasay, warshaddeennu waxay horumarinaysay alaabada heerka koowaad ee adduunka iyadoo la raacayo mabda'a
tayo leh marka hore. Alaabooyinkayagu waxay sumcad fiican ka heleen warshadaha iyo aaminaad qiimo leh oo ka dhexaysa macaamiisha cusub iyo kuwii horeba ..