Wax-soo-saar degdeg ah oo sarreeya: 200 μg-1.5 mg oo ah plasmid DNA ayaa soo saarta 1 saac oo leh saami aad u sarreeya oo ah qaab dhismeed aad u badan.
Urity Daahirsanaanta sare: DNA-ga plasmid-ka ah ee aadka u sarreeya waxaa helay nidaamka keydinta gaarka ah iyo Spin Column CP6.
Efficiency Wax -ku -oolnimada gudbinta oo aad u fiican: Ku habboon tijaabooyinka gudbinta ee khadadka unugyada badankood.
Applications Codsiyo baaxad leh: Ku habboon dheef -shiidka enzyme -ka xaddidaadda, beddelidda, isku xigxiga, microinjection, aamusnaanta hiddaha iyo tijaabooyinka gudbinta.
Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)
Soosaarid plasmid nuqul sare/hoose PBR322 plasmid-nuqul hooseeya, oo laga soo saaray 200 ml dhaqanka bakteeriyada iyadoo la adeegsanayo EndoFree Maxi Plasmid Kit, ayaa lagu xardhay 1 ml Buffer TB, oo leh 0.6 μg/μl. PBS plasmid-nuqul-sare ah, oo laga soo saaray 100 ml dhaqanka bakteeriyada iyadoo la adeegsanayo EndoFree Maxi Plasmid Kit, ayaa lagu xardhay 1 ml Buffer TB, oo ay ku urursan tahay 1.2 μg/μl. MIV: TIANGEN DNA Marker IV pBR322: 2 μl, pBS: 2 μl |
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pEGFP oo laga helay EndoFree Maxi Plasmid Kit ayaa si gooni ah loogu gudbiyay khadka unugyada endotoxin-dareen-daran 293T iyo unug-xasaasiyad-xasaasiya endotoxin unugyada Huh7 ee TIANGEN TIANfect. Muujinta GFP waxaa lagu ogaadey 24 saac kadib gudbinta. |
Tan iyo markii la aasaasay, warshaddeennu waxay horumarinaysay alaabada heerka koowaad ee adduunka iyadoo la raacayo mabda'a
tayo leh marka hore. Alaabooyinkayagu waxay sumcad fiican ka heleen warshadaha iyo aaminaad qiimo leh oo ka dhexaysa macaamiisha cusub iyo kuwii horeba ..