■ Way fududahay in la isticmaalo: Hawlgalka softiweerka ayaa si fudud loogu hanan karaa 1 h. Barnaamijka diyaarinta PCR/qPCR ee la dhisay waa la beddeli karaa oo degdeg baa loo gudbin karaa.
Consum Waxyaalaha la istcimaali karo: Caaradda dhuumaha lagu beddeli karo ee leh nidaamka Beckman Biomek 3000.
Maintenance Dayactir fudud: Module -ka tuubbada otomaatiga ah (APM) si fudud ayaa loo beddeli karaa oo dib loogu celin karaa si loo saxo.
Accuracy Saxnaan iyo saxnaan sare.
Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)
Jaantus 1: Natiijooyinka qalooca caadiga ah ee qPCR waxay muujinayaan ku -celcelin wanaagsan Tijaabooyinka cDNA ee 7 μl NIH 3T3 unugyo ayaa lagu qasi jiray 4 jeer 21 μl oo biyo ah marka loo eego saamiga 1: 4. Roche LightCycler 480 qalabka PCR-ga ee fluorescence-waqtiga dhabta ah iyo SuperReal PreMix Plus (SYBR Green) ayaa loo adeegsaday ogaanshaha. |
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Jaantuska 2: Saxnaanta sare marka la barbar dhigo dhuumaha gacanta (Bidix: Buugga; Midig: TEasy Pipetting Automated) 4 soo noqnoqoshada weyneynta GAPDH ee aadanaha (qalooca sare). Ku dar 2 μl cDNA ilaa 18 μl MasterMix si loo sameeyo nidaamka falcelinta 20 μl. Roche LightCycler 480 aaladda PCR-ga ee cabbirka fluorescence-waqtiga dhabta ah iyo SuperReal PreMix Plus (SYBR Green) ayaa loo adeegsaday ogaanshaha. |
Tan iyo markii la aasaasay, warshaddeennu waxay horumarinaysay alaabada heerka koowaad ee adduunka iyadoo la raacayo mabda'a
tayo leh marka hore. Alaabooyinkayagu waxay sumcad fiican ka heleen warshadaha iyo aaminaad qiimo leh oo ka dhexaysa macaamiisha cusub iyo kuwii horeba ..