Protection Ilaalin hufan: Ka ilaali RNA -ga dhiiga cusub wasakhda.
■ Ku habboon: Qalliinku wuxuu qaadanayaa 2 tallaabo oo keliya wuxuuna ku habboon yahay kaydinta tiro badan oo dhiig ah.
Compat Is -waafajin dhammaystiran: Waxay la jaanqaadi kartaa TIANGEN's Silicon Matrix Membrane Nits Kit si loo soo saaro dhiigga RNA ama DNA si loo hubiyo dhalidda soo -saaridda iyo daahirnimada.
Dhammaan alaabooyinka waxaa loo habeyn karaa ODM/OEM. Faahfaahinta,fadlan guji Adeegga La -habeeyey (ODM/OEM)
Qalab: 100 μl dhiig cusub oo jiir ah Habka: Wadarta RNA ee dhiigga jiirka cusub (lagu kaydiyey RNALock Reagent) ee lagu keydiyey 15-25 ℃, 4-8 ℃, -20/-70 ℃ ayaa la go'doomiyey iyadoo la adeegsanayo RNAprep Pure Blood Kit (4992238) iyadoo la raacayo nidaamka RNALock . Natiijooyinka: Fadlan fiiri sawirka korontada jel agarose jel. 4-6 μl of 50 elul eluates ayaa lagu raray haad kasta. M: TIANGEN DNA Marker III; Lane 1-2 (xakamayn togan): RNA ayaa laga nadiifiyay dhiig cusub; Lane 3-4: RNA ayaa laga sifeeyay shaybaarrada dhiigga ee lagu keydiyey 15-25 ℃ muddo 2 maalmood ah. Lane 5-6: RNA ayaa laga sifeeyay shaybaarrada dhiigga ee lagu keydiyey 4-8 ℃ muddo hal toddobaad ah. Laynka 7-8: RNA waxaa laga sifeeyay shaybaarrada dhiigga ee lagu keydiyey -20 ℃ ama -70 ℃ muddo nus sano ah. Qalabka korontada ayaa lagu sameeyay 6 V/cm muddo 30 daqiiqo ah 1% agarose. |
Tan iyo markii la aasaasay, warshaddeennu waxay horumarinaysay alaabada heerka koowaad ee adduunka iyadoo la raacayo mabda'a
tayo leh marka hore. Alaabooyinkayagu waxay sumcad fiican ka heleen warshadaha iyo aaminaad qiimo leh oo ka dhexaysa macaamiisha cusub iyo kuwii horeba ..