Bishii Diseembar 2019, kiisas badan oo ah oof wareenka oo aan la garanayn sababta ayaa ka bilaabmay Wuhan, Gobolka Hubei, iyo ugu dhakhsaha badan ku fiday inta badan gobollada iyo magaalooyinka Shiinaha, iyo dalal kale oo badan Janaayo 2020. Laga bilaabo 22:00 pm 27-kii Janaayo, 28 kiis oo la xaqiijiyay iyo 5794 la tuhunsan yahay 2019-nCov ayaa laga soo sheegay Shiinaha. Theilaha caabuqa ee fayraska ayaa loo fahmay sida Rhinolophus, heerka dhimashada hadda waa 2.9%.
Janaayo 12, 2020, Ururka Caafimaadka Adduunka (WHO) wuxuu ku dhawaaqay in cudurka sambabka uu sababay nooc cusub oo coronavirus ah (2019-nCov). Coronavirus waa nooc fayras ah oo ku faafa xayawaanka. Nooca fayraska nucleic acid waa hal RNA oo xanniban. Isla mar ahaantaana, WHO ayaa sidoo kale sii deysay macluumaadka isku xigxiga ee aaladda nucleic acid ee nCov oo ay wadaageen aqoonyahanno Shiineys ah, kaas oo bixiyay saldhigga ogaanshaha molikula ee ogaanshaha fayraska isla markaana suurtageliyay ogaanshaha degdegga ah iyo ogaanshaha cudur -sidaha.
WHO waxay soo jeedinaysaa in dadka qaba cudurrada neef -mareenka ee daran sida qufaca, qandhada, caabuqa mareenka neef -mareenka oo ay 14 maalmood gudahood ku joogeen Wuhan ama ay la kulmeen inay la xiriiraan bukaannada kale in si buuxda loo baaro. 17-kii Janaayo 2020, WHO ayaa sii deysay “Tijaabada shaybaarka ee coronavirus-ka cusub ee 2019 (2019-nCoV) ee kiisaska aadanaha ee la tuhunsan yahay, hagid ku-meelgaar ah, 17-ka Janaayo 2020”. Tilmaamuhu wuxuu tilmaamayaa in muunadaha laga uruurinayo bukaannada astaamaha leh ay ka mid yihiin tijaabooyinka neefsashada (nasopharynx iyo oropharyngeal swabs, xaako, lavage bronchoalveolar, iwm) iyo tijaabooyinka serum, sida soo socota:
*Si aad u qaaddo shaybaarka si aad u ogaato fayraska, isticmaal VTM (dhexdhexaadinta fayraska) oo ay ku jiraan daawooyinka antifungal iyo antibiotics. Dhaqanka bakteeriyada ama fangaska, gaadiid qalalan ama qadar aad u yar oo biyo nadiif ah. Iska ilaali qabowga soo noqnoqda iyo dhalaalinta muunadaha.
Marka laga soo tago agabyada ururinta gaarka ah ee lagu tilmaamay jadwalka sidoo kale hubso agabyo kale iyo qalab ayaa la heli karaa: tusaale weelasha gaadiidka iyo bacaha ururinta muunadaha iyo baakadaha, qaboojiyaha iyo baakadaha qabow ama barafka qalalan, qalabka dhiiga ee madhalayska ah (tusaale cirbadaha, irbadaha iyo tuubooyinka), sumadaha iyo summadaha joogtada ah, PPE, agabka wasakhda meelaha dushiisa.
Si loo ogaado fayraska, tijaabooyinka serum -ka waxaa loo isticmaali karaa in lagu ogaado fayraska habka difaaca jirka, halka RTqPCR lagu talinayo in lagu ogaado nCov nucleic acid si loo helo dhaqso oo sax ah. Sida fayraska
isku xigxiga waa la og yahay, waxa ku jira fayraska nucleic acid ee muunadda waxaa si dhakhso ah lagu ogaan karaa oo keliya
xulashada reagentyada habboon iyo raasamaalka u dhigma
(Ogaanshaha ogaanshaha Wuhan coronavirus 2019 ee waqtiga dhabta ah RTPCR, Baratakoolka iyo qiimeynta horudhaca ah laga bilaabo Jan 13, 2020) Hoggaamiye ahaan dhinaca beerista soo saarista aashitada nucleic acid iyo ogaanshaha Shiinaha, TIANGEN BIOTECH (BEIJING) CO., LTD. wuxuu siiyay fayraska afka-afka-gacmeed iyo hargabka A (H1N1) reagents-ka wax ka ogaada fayraska in ka badan 10 milyan oo qof. Sannadka 2019, fayras -ka TIANGEN ee soo -saaridda nukleerka asiidhka iyo soo -saareyaasha wax -soo -saarka ayaa lagu dabaqay 30 milyan oo muunado qandho -doofaar ah oo heer -sare ah oo Afrikaan ah, oo kaalin weyn ka qaatay ogaanshaha iyo ka -hortagga qandhada doofaarka qadiimiga ah ee Afrika ee Shiinaha. TIANGEN kaliya ma bixiso soosaarista fayraska deg deg ah oo sax ah iyo soo -saareyaasha ogaanshaha, laakiin sidoo kale waxay bixisaa aalado wax ku -shiidh unugyo ku habboon oo hufan iyo soo -saareyaasha nukliyeerka otomaatiga ah, kuwaas oo ka kooban xalka dhammaystiran ee soo -saarista fayraska iyo ogaanshaha.
Xalka Soo -saaridda Nucleic Acid ee Tooska ah
TIANGEN Soosaarayaasha Awoodda Nucleic Acid -ka ah waa aalado otomaatig ah oo loogu talagalay soo -saaridda aashitada nukliyeerka iyadoo la adeegsanayo habka kuusha magnetic. Codsiga aaladahaani ma aha oo kaliya inuu si weyn u yareeyo culeyska shaqada ee kormeerka iyo waaxaha karantiilnimada, laakiin sidoo kale wuxuu yareeyaa khaladaadka hawlgalka ee soo saarista gacanta, wuxuuna hubiyaa joogtaynta iyo xasilloonida tayada nucleic acid la soo saaray.
TIANGEN Automatic Nucleic Acid Extractor waxay leedahay marinno kala duwan (oo ay ku jiraan 16, 24, 32, 48, 96 kanaal), iyo reagents -ka is -waafajinta waxaa loo adeegsan karaa soo -saaridda aashitada nukliyeerka noocyo kala duwan oo muunad ah. TIANGEN sidoo kale waxay bixisaa horumarinta reagent loo habeeyay iyo adeegyada dhexgalka aaladda sida waafaqsan shuruudaha tijaabada ee kala duwan.
TGrinder H24 Homogenizer Cadka
Si aad ah loogu isticmaalo shiididda iyo isugeynta unugyada kala duwan iyo saambarada saxarada
● Iyada oo la shiidayo xoog isku-dhafan oo ah 2-5 jeer ka badan kuwa qalabka dhaqanka
Grin Isku -shiidid isku mid ah iyo isku -midaynta, ka fogaanshaha faddaraynta iskutallaabta
Device Qalab ilaalin otomaatig ah si loo ilaaliyo badbaadada shaqaalaha sheybaarka
TGuide S32 Automatic Nucleic Acid Extractor
● Muunad-soo-saarka: 1-32 muunado
Volume Habka socodsiinta: 20-1000 μl
Type Nooca muunadda: Dhiigga, unugyada, unugyada, saxarada, fayraska iyo muunado kale
Time Wakhtiga hawsha: Illaa 8 daqiiqo si aad u hesho fayraska nucleic acid
Mode Habka xakamaynta: Habka xakamaynta laba -geesoodka ah ee Windows Pad iyo badhanka shaashadda
Development Horumarka madal: Madal furan, oo lacag la'aan ah si ay ula jaanqaado reagents
● TGuide S32 Magnetic Viral DNA/Kit RNA (DP604)
Application Codsi ballaadhan: Fayraska DNA-ga oo tayo sare leh ayaa laga nadiifin karaa serum, plasma, sambal suuf, xalka daaweynta unugyada iyo xalalka dhowrka fayraska ee kala duwan.
● Fudud oo hufan: Badeecadani waxay si fiican ugu habboon tahay TGuide S32 Automatic Nucleic Acid Extractor, kaas oo soo saari kara fayraska DNA/RNA oo leh wax -soo -saar sare, daahirnimo sare, tayo deggan oo lagu kalsoonaan karo.
Codsiyada hoose: Nucleic acid-ka la nadiifiyey ayaa ku habboon tijaabooyinka hoose ee fayraska PCR iyo PCR-ga waqtiga-dhabta ah.
Buugga Xakamaynta Nucleic Acid Buugga
Hoggaamiye ahaan gudaha soo -saaridda iyo soo -ogaanshaha asiidhka nucleic acid, TIANGEN waxay leedahay taxanaha ugu dhammaystiran ee alaabooyinka soo -saaridda nucleic acid ee Shiinaha, kuwaas oo ku habboon ogaanshaha coronavirus -ka nooc kasta oo shaybaar ah: dhiig, serum/plasma, unug, suuf, fayras , iwm.
TIANamp Virus DNA/RNA Kit (DP315)
Efficiency Waxqabad sare: Fayraska DNA -ga oo tayo sare leh iyo RNA waxaa lagu heli karaa nadiifin degdeg ah oo leh soo noqnoqosho sare.
Urity Daahirsanaanta sare: In gebi ahaanba laga saaro wasakheynta iyo ka -hortagayaasha arjiga hoose.
Safety Nabadgelyo sare: Soo -saaridda reagent dabiici ah ama roobab ethanol looma baahna.
Xalka Ogaanshaha Fayraska RNA
1. TEasy Automatic Pipetting System
Accuracy Saxnaan sare: Barkinta qaboojintu waxay ku hayn kartaa heerkulka saamiga reagent ka hooseeya 7 ℃ in ka badan 60 min. APM kasta waxaa loo habeeyay si waafaqsan heerarka caalamiga ah, kaasoo leh saxnaan ka sareysa dhuumaha gacanta.
Operation Qaliin fudud: Cabbir yar. Miisaan fudud. Looma baahna qalab loogu talagalay dadka beddelaya xannibaadda. Dhisay PCR/qPCR habka diyaarinta. Hawlgalka gacanta ee la fududeeyay ee diyaarinta xalka PCR.
Application Codsi ballaaran: Waxaa loo adeegsan karaa dhuumaha saxanka 96/384, PCR, qPCR, ogaanshaha hiddaha iyo tijaabooyin kale oo heer sare ah.
FastKing One Step RT-qPCR Kit (Baaritaan) (FP314)
Aaladda FastKing One Step RT-qPCR Kit (Baaritaan) (FP314) oo ay soo saartay TIANGEN waa xirmo wax lagu ogaanayo tirooyinka wax-ka-beddelka ah ee gadaal-ka-noqoshada gadaal-ka-noqoshada oo ku saleysan habka baarista, kaas oo si gaar ah loogu talagalay in lagu ogaado hidde-raacayaasha shay-baarka kala duwan. KingRTase ee ku jira xirmada ayaa ah transcriptase -ka cusub ee molecular modified transcriptase, kaas oo leh isku xirnaanta RNA iyo xasilloonida kuleylka, oo leh hufnaanta qoraalka qoraalka oo la hagaajiyay iyo awoodda fidinta ee qaab -dhismeedka RNA ee qaab -dhismeedka labaad ee adag. Bilow kulul oo cusub Taq DNA polymerase ayaa sidoo kale la adeegsadaa si loo siiyo falcelinta PCR wax ku oolnimada sare u qaadista iyo gaar ahaanshaha. Intaa waxaa sii dheer, xirmadu waxay u fududeyneysaa qeybaha ilaa inta ugu badan iyadoo lagu darayo Taq iyo MLV enzyme isku-darka enzyme, iyo isku-darka ka-hortagga ion-ka, dNTPs, dejinta PCR iyo kor-u-qaadista MasterMix, si tallaabooyinka isku-darka qaybaha badan ay u noqon karaan
la fududeeyay.
Efficiency Wax -ku -oolnimada sare: Dib -u -noqoshada aadka u fiican iyo polymerase DNA waxay hubiyaan wax -ku -oolnimada falcelinta sare
Revers Dib -u -noqosho wanaagsan: Polymerase -ku wuxuu ka akhrisan karaa shaxanka RNA oo leh GC sare iyo qaab dhismeed labaad oo adag
Applications Codsiyo ballaadhan: Codsi sare oo loo adeegsan karo qaab -dhismeedka RNA oo leh wasakh ka timaadda noocyo kala duwan
Sens Dareenka sare: Istaraatiijiyadaha sida ugu hooseeya 1 ng ayaa si sax ah loo aqoonsan karaa, gaar ahaan shaxannada tirada badan
Tusaale Raadinta Fayraska RNA
Nucleic acid ee hargabka avian H5 waxaa soo saaray TGuide S32 Magnetic Viral DNA/RNA Kit (DP604). Qalabka FastKing One Step RT-qPCR Kit (Baaritaan) (FP314) ayaa loo isticmaalay ogaanshaha RT-qPCR iyadoo la adeegsanayo H5 khaanadaha fayraska hargabka avian iyo baaritaannada.
ABi7500Fast waxaa loo adeegsaday ogaanshaha RT-qPCR. Natiijooyinka H5 fayraska fayraska hargabka ee antigen (10-2, 10-3, 10-4, 10-5, 10-6and 10-7 dilution) oo laga soo qaaday 200 μl ayaa muujinaya wax-soo-saaridda fayraska nukliyeerka sare ee fayraska, kaas oo la kulmi kara kuwa xiga Baahiyaha dib-u-qorista, PCR, RT-PCR, PCR-waqtiga-dhabta ah, iwm PCR-ga waqtiga-dhabta ah wuxuu keenaa natiijooyin leh xasaasiyad sare, ku-celcelin wanaagsan iyo isku-dheellitirnaan khafiif ah. Isku -uruurinta kala duwan ee shaybaarka asiidhka fayruuska si sax ah ayaa loo ogaan karaa.
Waqtiga dhejinta: Apr-11-2021
